The Cube Reader is a small, but very advanced on-site Diagnostic Machine which is programmed by a Kit and Lot-specific Calibration Card.
The SAME Cube Reader when paired with the Equine/Camelid Adapter can be used to obtain on-site digital number results for
▪ Immuno-chek IgG Foal IgG (Equine) + Calf and Cria IgG (Camelid)
▪ SAA-chek Serum Amyloid A (SAA)
▪ Quick P4 Equine Progesterone
The SAME Cube Reader when paired with the Canine Adapter can be used to obtain on-site digital number results for
▪ Quick P4 Canine Progesterone
The IgG, SAA & Progesterone kits all have:
▪ 10 or 15 minute Number Results
▪ Room Temperature Stable
▪ Multiple 2+ Year Shelf Life
▪ Cost effective
The Test Cassette, which includes all the dry reagents, is positioned next to the Cube Reader using a special adapter.
The optical electronic sensors take multiple high resolution scans of the Test line intensities in order to get accurate results.
Using the Test line intensities and the mathematical information on the Calibration card, the Cube Reader calculates and displays the concentration.
The Cube Reader times the reaction for 10 or 15 minutes and saves the Last Reading.
Troubleshooting the Cube:
The Cube is a mini computer.
Rebooting the Cube is one of the quickest ways you can fix an issue with the machine.
Any unusual behavior, such as an error, failure to read a card, low battery, a result that is invalid or clearly wrong can mean that a Reboot of the Cube is needed.
To do this remove it from its power source, remove the battery and/or unplug the cable for at least 30 seconds.
A simple restart refreshes the system memory making it ready to start over again.
Our Equine Quick P4 Test Compared with Cornell Lab and BET Lab RIA( Radioimmunoassay)
The calculation is the result of multiple photos (data points). Both test and control lines are used in the interpolation of the stored Standard Curve. The darkest part of the test and control lines are used in calculation with background correction.
Table 1. Quick P4 vs. lab results for pregnant mares.
| Lateral Flow Quick P4 (ng/ml) |
RIA Cornell Lab (ng/ml) |
RIA BET Lab (ng/ml) |
Horse ID | Days Pregnant | Owner Action |
|---|---|---|---|---|---|
| 8.6 | 6.5 | 5.5 | "Cat" | 95 | |
| 6.4 | 6.0 | 5.5 | "Remedy" | 24 | |
| 5.7 | 8.8 | 66 | 31 | No supplementation | |
| 3.3 | 8.7 | 6.5 | 90 | 48 | No supplementation |
| 15.0 | 4.8 | 4.5 | 21 | 250 | No supplementation |
| 9.6 | 30.8 | 25.2 | 102 | 137 | No supplementation |
| 3.7 | < 0.06 | "Martha" | 244 | No supplementation (lab is wrong, mare is pregnant) |
|
| 4.6 | 5.7 | 88 | 282 | Regu-Mate® | |
| 4.0 | 3.9 | 110 | 310 | Regu-Mate® | |
| 3.2 | 7.1 | 115 | 249 | Regu-Mate® | |
| 9.3 | 16.6 | 18.7 | 82 | 66 | Regu-Mate® + P4 Oil |
| 5.5 | 9.7 | 8.8 | 54 | 31 | Regu-Mate® + P4 Oil |
| 4.7 | 5.3 | 105 | 190 | Regu-Mate® + P4 Oil |
Table 2. Quick P4 vs. lab results for non-pregnant mares.
| Lateral Flow Quick P4 (ng/ml) |
RIA Lab (ng/ml) |
Horse ID | Reproductive Status |
|---|---|---|---|
| 1.1 | 0.6 | 866 | Not Pregnant |
| 5.6 | 11.3 | Y46 | Not Pregnant |
| 2.5 | 0.2 | "Vodka" | Not Pregnant |
| 0.2 | 0.5 | 32 | Foaled 3 days prior |
Notes:
- Lateral flow test has 2 specific monoclonal antibodies to bind P4 in a sandwich assay.
- RIA (Radioimmunoassay) lab tests use a single polyclonal antibody to bind P4 - in competitive assay - this results in less specific binding
- RIA lab results may give higher progesterone values which include both progesterone and progesterone metabolites.
