Breed with confidence.

Quantitative numerical results in minutes.

You want healthy puppies and to keep danger at bay! Accordingly, you'll need to monitor your dog’s progesterone levels to determine the proper C-section timing. With one easy, on-site test, you will be able to assess progesterone levels to accurately predict ovulation and target optimal breeding dates and accurately time a C-section (when progesterone drops before whelping).

TargetVet canine progesterone testing


Our diagnostic products provide important data on the canine reproductive cycle including:

  • Our Canine Cube Reader generates numerical test results in just 15 minutes.
  • Easy to use, on-site testing.
  • Result lines are visible to the eye for easy checking.
  • Our test kits are stable at room temperature and have long shelf life.

Canine Birth and C-Section Timing

Predicting Ovulation is done by accurately detecting the initial rise in progesterone. Ovulation usually occurs 2 days after the initial rise in progesterone. The most fertile time to breed is 2 to 4 days after ovulation.

To time C-Section or whelping, test progesterone starting 58 – 60 days after first breeding (average gestation 63 days). A drop in progesterone to < 2 ng/mL indicates whelping will occur within 24 – 36 hours.

TargetVet canine cube reader

Why C-Section Timing is Important

Determination of progesterone is very important in the Canine reproductive cycle in deciding when to breed or when to do a C-section.

Canine cycles are variable, progesterone can rise slowly or very quickly (weekends and holidays included). Progesterone often varies from one cycle to the next and physical signs can be misleading.

Because of the importance of knowing and planning action based on progesterone level information, the delay in getting laboratory results is best avoided. Also, doing some testing at home is less stressful to the animal and its owner.

How it Works

The concentration of progesterone in the fresh sample is related to the color intensity of the test line. All the reagents are immobilized on the cassette and only Sample is added to get the result.

Because progesterone concentration is very low (parts per billion) (ng/ml), an optical reader is required to convert the line intensities to concentration numbers.

The Cube Reader digitizes the line intensities and accurately calculates the progesterone concentration based on a standard curve programmed for the lot. For every production lot of test cassettes, multiple samples are tested and a standard curve is determined and stored on a memory card.

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