TargetVet FAQ

Frequently Asked Questions

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Customer Policies & Support

What is your shipping policy?
All diagnostic kit prices include the cost of shipping. We ship via FedEx 2-Day service, ensuring delivery within 2 business days throughout the United States. Orders placed after 3 PM EST (Monday–Friday) will be processed the next business day. Weekend orders will be processed the following business day (typically Monday unless it's a holiday).
Do you offer expedited shipping?
Yes, we offer an Overnight Delivery option for an additional shipping fee. This ensures your order is delivered the next business day after processing.
Do you offer international shipping?
Currently, we do not offer international shipping through our website store. However, we partner with international distributors who provide our products in their respective markets. For international orders placed directly with us, a minimum of 5 test kits is required.
Do you offer discounts for distributors or bulk orders?
Yes, we offer discounts for distributors and bulk orders! Discounts start with a minimum order of 5 test kits and increase based on the quantity ordered. For more details on our tiered pricing system and current distributor rates, please contact us directly.
Do you offer technical or customer support if I encounter issues with the kits?
Yes, we're here to help! Our team provides support over the phone and via email during business hours (Monday–Friday). Please don't hesitate to reach out if you need assistance.
Can I cancel or modify my order after it's been placed?
If your order hasn't been processed yet, we'd be happy to assist with modifications or cancellations. Please contact us as soon as possible, and we'll take care of it.
What is your return and refund policy?
We accept returns for undamaged and unopened items within 30 days of purchase. Returns made after 7 days are subject to a 25% restocking fee.
Do you work with educational facilities like colleges and universities?
Yes! We love supporting educational courses and are happy to offer discounted test kits to educators. Please reach out to us with details about your research or class, and we'll work to meet your needs. At a minimum, we offer a 25% discount on orders for educational purposes.

Immuno-Chek G Foal IgG Test Kit

What is the shelf life of the test kit?
The test kit is valid for 12 months from the manufacturing date.
Can I reuse the pipettes?
No, the pipettes are single-use only.
Which tube do I use to collect the sample for the IgG test?
BioMetallics recommends collecting a 1–2 ml anticoagulated blood sample using a heparin or EDTA tube.
How should I store the IgG kit?
The entire kit and components should be stored at room temperature in order to maintain the labeled shelf life.
What are some factors that can lead to inaccurate IgG results?
  • The pipette may not be working correctly, causing more than 10 µL of whole blood to be drawn.
  • There is excess blood on the outside of the pipette tip.
  • Not enough mixing of the sample.
  • Adding less than 3 drops of Dropper Bottle B solution. If fewer than 3 drops of buffer are added, there will not be enough buffer to run the sample through the membrane — the Test and Control Lines may not develop correctly, making the test invalid.
How do I know if something went wrong when running the IgG test?
If you are unsure about the results or have problems comparing the line intensities with the Results Interpretation Chart, you can take a picture of the cassette and call or email TargetVet/BioMetallics right away — we'll be happy to help. IgG test result interpretation example
I ran the same samples with the Immuno-Chek G Foal IgG Test and another test and got different results. Why?

Two different kinds of tests cannot be compared directly, so slightly different results are expected.

The Immuno-Chek G foal IgG test is a sandwich-type Lateral Flow (LF) test. Two specific antibodies for equine IgG are placed on the membrane and bind the IgG in the sample. Our antibodies do not cross-react with molecules such as albumin or fibrinogen, which increases accuracy and reliability. Cross-reactivity introduces large errors in turbidimetric and other assays — albumin and fibrinogen both aggregate during IgG measurement, giving erroneously higher values.

Once the equine IgG antibodies bind, the Cube Reader digitizes the line intensities and accurately calculates IgG concentration based on a standard curve programmed specifically for that production lot.

What is the sensitivity and specificity of the Immuno-Chek G Foal IgG Test?
TargetVet/BioMetallics has done extensive in-house testing. Sensitivity for Equine IgG is 99.5% and Specificity is 96%, based on analysis of 65 IgG samples.
How does the Cube Reader calculate Foal IgG concentration?
The optical electronic sensor takes multiple high-resolution scans of the test and control line intensities. Using those intensities and the standard curve on the calibration card, the Cube Reader calculates and displays the IgG concentration (mg/dL). Equine standards and controls are used to optimize every lot of Foal IgG.

Equine Quick P4 Progesterone Test Kit

What is the correct procedure to run the Equine Quick P4 test?

Collect the blood in a plasma tube (green or purple). Mix by inverting the sample tube several times. Wait 1 hour between sample collection and running the test.

After collecting the sample, you may either:

Option A: Spin down at 2,500 rpm, then place the sample in the refrigerator and wait 1 hour before testing. Use the clear plasma for testing.

Option B: Keep the sample in a rack for 1 hour, then spin down at 2,500 rpm. Use the clear plasma for testing.

Why is it necessary to wait at least one hour to run the sample?
The viscosity of the sample changes over time — the lower the viscosity, the faster it runs on the cassette. The test is optimized for samples between one hour and two days old. To ensure the sample follows our standard curve accurately, it must be at least one hour old.
What does it mean that the test results correspond to the free (active) progesterone present in sera?
Progesterone in the blood exists as either a free circulating molecule or a protein-bound molecule. The free (unbound) progesterone is the biologically active form responsible for the mare's cycle and reproduction. The Equine Quick P4 test detects only this biologically active progesterone in a plasma sample.
What is the minimum progesterone level to maintain pregnancy?
The minimum safe level to maintain pregnancy is ≥ 4 ng/mL.
Why am I getting a progesterone concentration of 1–3 ng/mL if the mare is pregnant?
A result below 1 ng/mL indicates a progesterone-deficient mare. Mares with progesterone concentrations below 4 ng/mL may be at increased risk of pregnancy loss.
When should I test progesterone to monitor pregnancy?
To determine if progesterone levels are adequate to maintain pregnancy, test a plasma sample 12–14 days after ovulation. Retest at various intervals during pregnancy to ensure adequate levels are being maintained.
When should I test for pregnancy determination?

To detect pregnancy, test 21–23 days after breeding. The progesterone concentration should be ≥ 5 ng/mL.

Note: Quick P4 indicates the presence of progesterone, not specifically pregnancy. A positive result should be confirmed by palpation or ultrasound.

What are some factors that can lead to inaccurate QP4 results?
  • More than one drop of sample is added to the cassette.
  • Adding fewer than 3 drops of Dropper Bottle B solution. Without enough buffer, the sample cannot properly run through the membrane — the Test and Control Lines may not develop correctly, making the test invalid.
How do I know if something went wrong when running the QP4 LFA test?

Signs of a problem include:

  • Control line is not visible.
  • Lines are faint or not present.
QP4 test result interpretation example

If you're unsure about results, take a photo of the cassette and call or email TargetVet/BioMetallics right away — we'll be happy to assist.

What is the sensitivity and specificity of the Equine Quick P4 Test?
Sensitivity is 93.5% and specificity is 95.2%, based on analysis of 72 mare samples.
TargetVet/BioMetallics has two tests that measure Equine Progesterone. How do I know which is better for me?

We sell two tests for equine progesterone:

Target Equine Progesterone — A semi-quantitative test that shows shades of blue to white, indicating a range of 0–5 ng/mL. Uses serum or plasma. Pregnancy should be maintained above 3 ng/mL. Sold in 6 or 12 test quantities. Best if you only need occasional tests and prefer a simple visual result.

Equine Quick P4 — A lateral flow test requiring the Cube Reader, giving a numerical result from 0–35 ng/mL. The Cube Reader is a one-time purchase; kits come in 5, 10, or 15 test refills. Best if you run multiple tests regularly and prefer precise numerical results.

We also offer IgG and SAA tests in a "Visual" (semi-quantitative) configuration for customers who prefer not to purchase a Cube Reader. Feel free to contact us if you need help deciding.

Target Test Kits (Equine & Canine)

How does the Target Test work?
The Target test is an ELISA test. A highly specific antibody binds the progesterone in the sample, then the target enzyme conjugate binds to the remaining empty antibody sites. A substrate is then added and reacts with the enzyme conjugate. After 9 minutes the reaction is complete and different shades of blue become visible. The lowest progesterone concentration produces the darkest blue; the highest concentration produces the least blue (white cup). Intermediate concentrations produce lighter shades of blue (C2 and C3 ranges).
What is the recommended shipping and storage for Target kits?
All kits are sent with 2-day shipping, which does not require ice or special packaging. However, customers should refrigerate the kits upon receiving them to maintain the 1-year shelf life.
Which tube do I use to collect sample for the Target test?
Collect the blood sample into a purple EDTA or heparin-coated tube for plasma, or a red-top tube for serum. Do NOT use gel separator type tubes.
Can I use whole blood to run the Target test?
No. Whole blood would clog the test and you would either get no blue color or an irregular splash of blue rather than a clean blue dot.
Do I need a centrifuge to get a plasma or serum sample?
You can either spin the blood down with a centrifuge for 10 minutes at 2,500 rpm, or allow the blood to clot at room temperature for ½–1 hour until clear serum collects in the top of the tube.
Why am I getting two shades of blue in the same test?
This is typically caused by poor washing technique or poor sample quality (hemolyzed or lipemic sample).
How do I interpret Target Color Results?
Always use the majority of the spot color to rate the result. A darker blue around the edge is due to poor washing (edges are always harder to wash). Accurate results require good quality, straw-colored sera/plasma. The canine test should be read at exactly 9 minutes — after that it may become darker blue or gray. Target test color result interpretation chart

Not sure about results? Take a photo and contact TargetVet/BioMetallics — we'll help you interpret it.

Can I use the Target Canine Ovulation kit to test equine progesterone?
No. The Target Canine Ovulation Kit is optimized for dogs only. We also sell a Target Equine Ovulation Kit specifically for horses.
What are the differences between the canine and equine Target tests?

1. Different progesterone concentration ranges:

Equine

  • Bright Blue (C1): 0–1 ng/mL
  • Light Blue (C2): 1–2 ng/mL
  • Faint Blue (C3): 2.1–5 ng/mL
  • White (C4): >5 ng/mL

Canine

  • Bright Blue (C1): <1 ng/mL
  • Light Blue (C2): 1–3 ng/mL
  • Faint Blue (C3): 3–10 ng/mL
  • White (C4): >10 ng/mL

Using the canine test on equine samples cannot distinguish between 3–5 and >5 ng/mL — a critical difference for pregnancy monitoring in mares.

2. Different viscosity: Canine samples are less viscous than equine, and each test is optimized for its species accordingly.

3. Different read time: Equine test is read at 7 minutes; canine test at 9 minutes.

No questions matched your search.

Try different keywords, or contact us directly — we're happy to help.

Still have a question? Reach us at sales@targetvet.com or 609-275-0133 (Mon–Fri during business hours).