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Two different kinds of tests cannot be compared directly, so slightly different results are expected.
The Immuno-Chek G foal IgG test is a sandwich-type Lateral Flow (LF) test. Two specific antibodies for equine IgG are placed on the membrane and bind the IgG in the sample. Our antibodies do not cross-react with molecules such as albumin or fibrinogen, which increases accuracy and reliability. Cross-reactivity introduces large errors in turbidimetric and other assays — albumin and fibrinogen both aggregate during IgG measurement, giving erroneously higher values.
Once the equine IgG antibodies bind, the Cube Reader digitizes the line intensities and accurately calculates IgG concentration based on a standard curve programmed specifically for that production lot.
Collect the blood in a plasma tube (green or purple). Mix by inverting the sample tube several times. Wait 1 hour between sample collection and running the test.
After collecting the sample, you may either:
Option A: Spin down at 2,500 rpm, then place the sample in the refrigerator and wait 1 hour before testing. Use the clear plasma for testing.
Option B: Keep the sample in a rack for 1 hour, then spin down at 2,500 rpm. Use the clear plasma for testing.
To detect pregnancy, test 21–23 days after breeding. The progesterone concentration should be ≥ 5 ng/mL.
Note: Quick P4 indicates the presence of progesterone, not specifically pregnancy. A positive result should be confirmed by palpation or ultrasound.
Signs of a problem include:
If you're unsure about results, take a photo of the cassette and call or email TargetVet/BioMetallics right away — we'll be happy to assist.
We sell two tests for equine progesterone:
Target Equine Progesterone — A semi-quantitative test that shows shades of blue to white, indicating a range of 0–5 ng/mL. Uses serum or plasma. Pregnancy should be maintained above 3 ng/mL. Sold in 6 or 12 test quantities. Best if you only need occasional tests and prefer a simple visual result.
Equine Quick P4 — A lateral flow test requiring the Cube Reader, giving a numerical result from 0–35 ng/mL. The Cube Reader is a one-time purchase; kits come in 5, 10, or 15 test refills. Best if you run multiple tests regularly and prefer precise numerical results.
We also offer IgG and SAA tests in a "Visual" (semi-quantitative) configuration for customers who prefer not to purchase a Cube Reader. Feel free to contact us if you need help deciding.
Not sure about results? Take a photo and contact TargetVet/BioMetallics — we'll help you interpret it.
1. Different progesterone concentration ranges:
Equine
Canine
Using the canine test on equine samples cannot distinguish between 3–5 and >5 ng/mL — a critical difference for pregnancy monitoring in mares.
2. Different viscosity: Canine samples are less viscous than equine, and each test is optimized for its species accordingly.
3. Different read time: Equine test is read at 7 minutes; canine test at 9 minutes.
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